R&D Systems leptin elisa experiment
Leptin Elisa Experiment, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp cxcl5 hs00607029 g1 (Thermo Fisher)

Thermo Fisher gene exp cxcl5 hs00607029 g1

Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).

Gene Exp Cxcl5 Hs00607029 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Journal:

Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide

doi: 10.1111/j.1365-2249.2009.03920.x

Figure Lengend Snippet: Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).

Article Snippet: RNA polymerase II (Hs00172187_ml) was used as an endogenous control. table ft1 table-wrap mode="anchored" t5 caption a7 Inflammatory mediator Primers and probe assay ID IL-1α (interleukin-1 alpha) Hs00174092_m1 IL-1β (interleukin-1 beta) Hs00174097_m1 IL-Ra (interleukin-1 receptor antagonist) Hs00277299_m1 IL-1R1 (interleukin-1 receptor-1) Hs00168392_m1 IL-1Acp (interleukin-1 accessory protein) Hs00370506_m1 ICE (IL-1 converting enzyme) (caspase-1) Hs00354836_m1 IL-18 (interleukin-18) Hs00155517_m1 IL-18R1 (interleukin-18 receptor) Hs00175381_m1 IL-18Acp (interleukin-1 accessory protein) Hs00187256_m1 IL-1F6 (interleukin-1 family 6) Hs00205367_m1 IL-1F7 (interleukin-1 family 7) Hs00367199_m1 IL-1F8 (interleukin-1 family 8) Hs00758166_m1 IL-1F9 (interleukin-1 family 9) Hs00219742_m1 IL-1F10 (interleukin-1 family 10) Hs00544661_m1 IL-1Rrp2 (IL-1 receptor-related protein 2) Hs00187259_m1 TNF-α (tumour necrosis factor alpha) Hs00174128_m1 TNFRSF1A (TNF alpha receptor superfamily 1A) Hs00236902_m1 TNFRSF1B (TNF alpha receptor superfamily 1B) Hs00153550_m1 TACE (TNF-α converting enzyme) Hs00234221_m1 IL-4 (interleukin-4) Hs00174122_m1 IL-6 (interleukin-6) Hs00174131_m1 OSM (oncostatin M) Hs00171165_m1 LIF (leukaemia inhibitory factor) Hs00171455_m1 OSMR (oncostatin M receptor) Hs00384278_m1 LIFR (leukaemia inhibitory factor receptor) Hs00158730_m1 IL-10 (interleukin 10) Hs00174086_m1 IL-12a (interleukin-12 chain a) Hs00168405_m1 IL-12b (interleukin-12 chain b) Hs00233688_m1 IL-32 (interleukin-32) Hs00170403_m1 GM-CSF (granulocyte macrophage-colony stimulating factor) Hs00171266_m1 IFN-γ (interferon gamma) Hs00174143_m1 CCL2 (monocyte chemottractant protein 1) Hs00234140_m1 CX3CL1 (fractalkine) Hs00171086_m1 CXCL5 (epithelial-derived neutrophil activating protein 78) (ENA-78) Hs00607029_g1 CCL5 (regulation on activation normal T cell expressed and secreted) (RANTES) Hs00174575_m1 CXCL8 (IL-8) Hs00174103_m1 CXCL10 Hs00171042_m1 Leptin Hs00174877_m1 LeptinR (leptin receptor) Hs00174497_m1 ADIPOR1 (adiponectin receptor-1) Hs00360422_m1 ADIPOR2 (adiponectin receptor-2) Hs00226105_m1 Visfatin Hs00237184_m1 INSR (insulin receptor) Hs00169631_m1 RAGE (receptor for advanced glycation end products) Hs00153957_m1 CD14 Hs00169122_g1 Control genes GAPDH (glyceraldehyde-3-phosphate-dehydrogenase) Hs99999905_m1 18S Hs99999901_s1 RNA pol II Hs00222679_m1 Open in a separate window Primers and probes for the quantification of gene expression using Taq man low-density arrays (TLDAs).

Techniques: Gene Expression, Activation Assay, Control

Escherichia coli lipopolysaccharide (LPS) upregulates differentially a subset of genes compared with Porphyromonas gingivalis LPS.

Journal:

Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide

doi: 10.1111/j.1365-2249.2009.03920.x

Figure Lengend Snippet: Escherichia coli lipopolysaccharide (LPS) upregulates differentially a subset of genes compared with Porphyromonas gingivalis LPS.

Techniques:

CXCL5 is differentially regulated by Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS) in myeloid cells. (a) Real-time reverse transcription–polymerase chain reaction analysis of CXCL5 expression using cDNA derived from THP-1 monocytes. Cells were treated with E. coli LPS (100 ng/ml) or P. gingivalis LPS (100 ng/ml) until the indicated time-points. Enzyme-linked immunosorbent assay was used to measure secreted CXCL5 in culture supernatants from (b) THP-1 monocytes, (c) peripheral blood monocytes and (d) monocyte-derived dendritic cells. The results were obtained from three independent experiments and are presented as average ± standard deviation.

Journal:

Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide

doi: 10.1111/j.1365-2249.2009.03920.x

Figure Lengend Snippet: CXCL5 is differentially regulated by Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS) in myeloid cells. (a) Real-time reverse transcription–polymerase chain reaction analysis of CXCL5 expression using cDNA derived from THP-1 monocytes. Cells were treated with E. coli LPS (100 ng/ml) or P. gingivalis LPS (100 ng/ml) until the indicated time-points. Enzyme-linked immunosorbent assay was used to measure secreted CXCL5 in culture supernatants from (b) THP-1 monocytes, (c) peripheral blood monocytes and (d) monocyte-derived dendritic cells. The results were obtained from three independent experiments and are presented as average ± standard deviation.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Expressing, Derivative Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

CXCL5 expression is not induced by the Toll/interleukin (IL)-1 receptor (TIR) domain-containing adaptor inducing interferon (IFN)-γ (TRIF)-biased Toll-like receptor (TLR)-4 ligand monophosphoryl lipid A (MPLA). THP-1 monocytes were treated with two different preparations of Escherichia coli lipopolysaccharide (LPS) (100 ng/ml), either strain 0111:B4 pure (black) or ultrapure LPS (white) or MPLA (1 µg/ml) (grey) for 24 or 48 h. (a) CXCL5 levels in culture supernatants following treatment with E. coli LPS or MPLA. (b) Tumour necrosis factor-α levels in the same culture supernatants following treatment with the above ligands. The results were obtained from three independent experiments and represent average ± standard deviation.

Journal:

Article Title: Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide

doi: 10.1111/j.1365-2249.2009.03920.x

Figure Lengend Snippet: CXCL5 expression is not induced by the Toll/interleukin (IL)-1 receptor (TIR) domain-containing adaptor inducing interferon (IFN)-γ (TRIF)-biased Toll-like receptor (TLR)-4 ligand monophosphoryl lipid A (MPLA). THP-1 monocytes were treated with two different preparations of Escherichia coli lipopolysaccharide (LPS) (100 ng/ml), either strain 0111:B4 pure (black) or ultrapure LPS (white) or MPLA (1 µg/ml) (grey) for 24 or 48 h. (a) CXCL5 levels in culture supernatants following treatment with E. coli LPS or MPLA. (b) Tumour necrosis factor-α levels in the same culture supernatants following treatment with the above ligands. The results were obtained from three independent experiments and represent average ± standard deviation.

Techniques: Expressing, Standard Deviation

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